LITE Switch: Light-inducible Gene Expression System
Control gene activation with light
LITE Switch is a light-inducible gene expression system for eukaryotic cells. This product is the first to allow users to regulate transcription of their gene of interest with light.
The system, first described in Motta-Mena et al., 2014, is based on EL222, a bacterial photosensor protein that binds specifically to a C120 DNA promoter when illuminated with blue light (450-495 nm) (Figure 1A).
VEL is a new, optimized version of EL222 that is now part of the LITE Switch System. Cells that express the VEL protein and contain a gene downstream of C120 promoter will express robust transcript levels, but only when cells are illuminated with blue light. In the dark, the system shows low basal transcriptional activity (<2-fold). The VEL transactivator provides a higher fold induction than the first generation VP-EL222 protein from Motta-Mena et al. (Figure 1B; 100-fold VP-EL222 vs. >300-fold VEL).
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LITE Switch Kit Contents
The LITE Switch Kit includes 20 ug of each of the following plasmids and a light source:
pVEL: regulatory plasmid for expression of the VEL transactivator protein
pC120: inducible expression plasmid contains the C120 light-inducible promoter and a downstream multiple cloning site
Light source: 12'' Blue LED light panel
LITE Switch Expression Kit
- Reversible induction is simplified by turning on or off of light source
- By spatially restricting the light source gene expression can be controlled with high resolution
- Reduced off-target effects compared to conventional chemical-inducible systems
- Large range of gene induction and low basal expression in both cell lines and living organisms
- VEL transactivator quickly activates (<10 sec) and resets (<50 sec) after illumination